SYS_EXTRACTION_INIT
DOWNLINKING VECTOR PARAMETERS
DECRYPTING CORE ALGORITHMS...
DECRYPTING CORE ALGORITHMS...
Audit your gene expression potential. Evaluate how well your DNA sequence is optimized for the translational machinery of the host organism.
Codon optimization involves more than just GC content; it requires matching the host's tRNA abundance and avoiding restrictive mRNA secondary structures. A low CAI score suggests that the sequence may suffer from poor translation rates and low protein yield.