SYS_EXTRACTION_INIT
DOWNLINKING VECTOR PARAMETERS
DECRYPTING CORE ALGORITHMS...
DECRYPTING CORE ALGORITHMS...
Audit the sequence. Calculate the melting temperature (Tm) of oligonucleotide primers for PCR, sequencing, and hybridisation based on the Wallace Rule and GC-content.
A Tm of 18.0°C suggests an optimal PCR annealing temperature (Ta) of approximately 13.0°C. For reliable amplification, ensure your forward and reverse primers have a Tm difference of less than 5°C.