SYS_EXTRACTION_INIT
DOWNLINKING VECTOR PARAMETERS
DECRYPTING CORE ALGORITHMS...
DECRYPTING CORE ALGORITHMS...
Audit the cut. Estimate the probability of Cas9 binding to unintended genomic sites based on guide RNA mismatch tolerance and total genome size.
A 20bp guide RNA is highly specific, but Cas9's tolerance for mismatches increases the risk of off-targets. With 2 mismatches permitted, there is a theoretical probability of 4.977 unintended sites in a 3.2B bp genome.