Genomics

🧬 DNA Melting Point (Tm) Auditor

Audit your primer thermodynamics. Calculate the melting temperature (Tm) using salt-adjusted empirical formulas.

Sequence Analysis

DNA Sequence (A, T, G, C)

Na+ Concentration (mM)

Calculated Tm

43.4°C

Length:16 bp

GC Content:50.0%

✓Confidence Score: High (Salt-Adjusted Wallace)

ℹSource:Samba et al. (1998) Empirical formulas for Tm

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