Molecular Biology

🧬 PCR Annealing Temp Auditor

Audit your thermal cycler settings. Calculate the optimal annealing temperature (Ta) to maximize specificity and yield.

Primer Metrics

Forward Primer Tm (°C)

Reverse Primer Tm (°C)

Polymerase Optimization

Optimal Annealing Temp (Ta)

57.5°C

Primer ΔTm:0.7°C

A ΔTm > 5°C may lead to asymmetric amplification or non-specific products. Consider adjusting primer length or GC content if ΔTm is too high.

✓Confidence Score: High (Standard PCR Heuristics)

ℹSource:Rychlik et al. (1990) Optimization of PCR

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